Part:BBa_K2066118:Design
Synthetic Enhancer with 3X TetO cassette (52s) on UNS backbone
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 111
Illegal NheI site found at 206 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 171
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 951
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We made this part to be compatible with the iGEM Biobrick standard as well as flank the synthetic enhancer circuit (Amit et. al. 2011) with UNS sequences for easy cloning.
Source
The enhancer, tet cassette, glnAp2 synthetic promoter, and NRI coding region sequences were derived from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). The UNS sequences at the ends of the insert are derived from Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!